Complement System AI Buffers
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Complement System AI Buffers
Compvide’s innovative buffers are designed to replace traditional barbital-based solutions used in scientific research. Our products ensure high-quality complement activation and fixation, mirroring the effectiveness of barbital without its regulatory drawbacks. With Compvide, researchers can confidently conduct experiments, knowing they are using a safe, compliant, and efficient alternative to regulated substances.
| Cat. No. | Name | Description | Volume | Price (USD) |
|---|---|---|---|---|
| CV0020 | Complement F-Buffer (No Ca, No Mg) | Assay foundation buffer | 1 L | $110 |
| CV0021 | Complement A/I Buffer (+ Ca, + Mg) | All Pathways Activation/inhibition Buffer | 1 L | $225 |
| CV0120 | Complement AP A/I Buffer (+ EGTA) | AP Activation/inhibition Buffer | 1 L | $247 |
| CV1020 | Complement 0/I Buffer (+ EDTA) | Inhibition Buffer | 1 L | $110 |
| CVD0020 | Complement HF-Buffer (No Ca, No Mg) | Hemolytic Assay Foundation Buffer | 1 L | $150 |
| CVD0021 | Complement A/I H-Buffer (+ Ca, + Mg) | All Pathways Hemolytic Assay Buffer | 1 L | $150 |
| CVD0120 | Complement AP A/I H-Buffer (+ EGTA) | Alternative Pathway Hemolytic Assay Buffer | 1 L | $170 |
| CVD1020 | Complement 0/I H-Buffer (+ EDTA) | Inhibition Hemolytic | 1 L | $150 |
| CV1021 | Complement LP A/0 Buffer (+ Ca) | Lectin Pathway special buffer | 1 L | Please inquire |
| CVT1020 | Complement LP A/0 W-Buffer (+ Ca) | Lectin Pathway special Wash buffer | 1 L | Please inquire |
Complement System A/I F-Buffer
CV0020
Complement System Foundation Buffer is a basic buffer that can be used to prepare:
1. Complement Activation/Inhibition Assay Buffer:
- To prepare this buffer, add both CaCl2 and MgCl2 to the basic complement system foundation buffer.
- This modified buffer is used for assays related to complement activation or inhibition.
2. Alternative Pathway Buffer:
- For an alternative pathway assay, add Mg/EGTA to the basic buffer.
- This specific buffer is designed to study the alternative pathway of complement activation.
3. Complement Stop Solution:
- To create a complement stop solution, add EDTA to the basic buffer.
- This solution is used to halt complement activation in experiments.
Instead of using the complement system foundation buffer to prepare other buffers, we highly recommend utilizing our ready-to-use, pathway-specific complement activation/inhibition buffers. These specialized buffers are designed for specific complement pathways, ensuring consistent and reliable results.
Complement A/I Buffer
CV0021
Complement AP A/I Buffer
CV0120
Our specialized buffer is tailored for assays targeting the complement system’s alternative pathway. It is precisely formulated with EGTA to sequester calcium ions, which are key to the classical and lectin pathways, ensuring exclusive activation of the alternative pathway. The buffer is also enriched with the right amount of magnesium chloride to facilitate this selective process.
Note:
This buffer comes in two parts, AP A/I Ba and AP A/I Bb, that should be mixed before use. Please see the package-enclosed instructions for details.
Complement 0/I Buffer
CV1020
This buffer is designed to inhibit all complement system activations. It contains EDTA, which chelates both calcium and magnesium ions in the serum, effectively blocking all pathways of the complement system.
Complement A/I HF-Buffer
CVD0020
Our Foundation Buffer is specially formulated to be isotonic and have low ionic strength, making it perfect for serum dilution or for cleansing erythrocytes and other target cells in complement-based in vitro studies.
Complement A/I H-Buffer
CVD0021
A/I H-Buffer is a D-glucose-enriched isotonic buffer with a reduced sodium content, is ideal for complement hemolysis assays. It supports the activation across all complement pathways and ensures cellular adherence at low ionic strengths.
Complement AP A/I H-Buffer
CVD0120
This buffer, akin to the AP A/I formulation, features a lower ionic strength and is enhanced with D-glucose to sustain ionic equilibrium. It’s specifically employed in hemolytic assays that are designed to initiate only the alternative pathway of the complement system.
Note:
This buffer comes in two parts, AP A/I H-Ba and AP A/I H-Bb, that should be mixed before use. Please see the package-enclosed instructions for details.
Complement 0/I H-Buffer
CVD1020
Buffers with EDTA are formulated to prevent activation of the complement system. These solutions, ideal for cell surface applications including hemolytic assays, are balanced with D-glucose and minimal NaCl to maintain the perfect isotonic conditions and ionic strength.
Complement LP A/0 Buffer
CV1021
The LP A/0 buffer is a hypertonic solution specifically designed for serum dilution in MBL/MASP activity assays. It effectively inhibits the binding of immune complexes, thereby preventing the activation of the classical pathway while preserving the integrity of the MBL/MASP complex.
Complement LP A/0 W-Buffer
CVT1020
The LP A/0 T buffer is formulated with a lower ionic strength than HSB, making it an ideal wash buffer for MBL/MASP activity assays. It effectively removes non-bound serum complement components, ensuring that the MBL/MASP complex stays securely attached to the target surface.
